Tingting Chen, Lihua Ning, Xu Liu, Dezhou Cui, Hua Zhang, Detao Li, Li Zhao, and Huabang Chen. Development of Functional Molecular Markers of I and IIb for the High Amylose Maize Germplasm Line GEMS-0067. Crop Science. 2013, 53: 482-490.

 

Abstract：The development of high amylose corn in the public sector has been hindered by the lack of high amylose germplasm available to breeders and difficulties in accurate and efficient amylose content measurement. GEMS-0067 is the first public germplasm that possesses recessive amylose-extender (ae) gene and modifier gene(s) that raises amylose level to over 70%. Several lines of evidences suggested that SbeI is one of the major modifier genes for high amylose corn, and our work for the first time showed that SbeI explained 49% of the total amylose variance in the amylose extender background. We developed and validated functional co-dominant molecular markers of SbeI and SbeIIb for GEMS-0067 that were able to distinguish AeAe, Aeae, and aeae genotypes. These simple, sequence-specific, PCR-based, low cost markers amenable to larger numbers of plant samples make them potentially viable for marker-assisted selection in practical high amylose corn breeding programs. GEMS-0067 was of mixed heterotic derivation. Our result clearly showed that GEMS-0067 was genetically more similar to Mo17 and was clustered into NSS heterotic group based on 25 SSR profiles. The information provided here would facilitate the development of high amylose corn.

 

文章链接：https://www.soils.org/publications/cs/view/first-look/c12-06-0386.pdf