设为首页  |  加入收藏  |  联系我们  |  English  |  中国科学院  
 首  页  实验室概况 研究队伍 科研工作 平台建设 开放交流 人才培养 规章制度
 
  当前位置:首页 > 最新论文  
Transient Expression of A TaGRF4-TaGIF1 Complex Stimulates Wheat Regeneration and Improves Genome Editing
 Fengti Qiu, Sinian Xing, Chenxiao Xue, Jinxing Liu, Kunling Chen, Tuanyao Chai & Caixia Gao


Science China Life Sciences
DOI:10.1007/s11427-021-1949-9


Abstract

 

Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site of TaGRF4, the resulting complex (mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of mTaGRF4-TaGIF1 together with a cytosine base editor targeting TaALS resulted in 2–9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, mTaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation.
 
【打印本页】【关闭本页】
Copyright © 植物细胞与染色体工程国家重点实验室(中国科学院遗传与发育生物学研究所)
地址:北京市朝阳区北辰西路1号院2号 邮编:100101 电话: 010-64806537 传真: 010-64806537